Optimized sparse sampling speeds up laser scanning microscopy!

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Courtney has developed a new strategy for improving the speed of laser scanning microscopy. The technique, called 3D Fast Acquisition Scan by z-Translating Raster (3D-FASTR), uses an electrically tunable lens (ETL) to generate a reproducible 3D sparse sampling pattern which fully and efficiently scans a volume in the fastest possible time without repeating until the volume is complete. This method has shown a 4-fold improvement in the volumetric imaging rate of live cells. The theory underlying 3D-FASTR is completely general and can be applied to any scanning imaging method. Find details in the recent publication in Optics Express!

Link: doi.org/10.1364/OE.27.036241